FIG 5.

Influence of the D109Y mutation on Asc1p-dependent phenotypes and Asc1p protein levels. The different ASC1 variants were expressed from CEN plasmids under the control of the native ASC1 promoter in the Δasc1 background. The ASC1 wild-type strain and the Δasc1 strain, both transformed with the empty vector (EV), were used as controls. (A) Resistance of the strains to cell wall stress (Congo red), osmotic stress (NaCl), low temperature (25°C), and inhibition of translation elongation (cycloheximide) was tested. (B) Haploid adhesive growth of the mutant strains on 3-AT. (C) Immunodetection of Asc1p within cell extracts obtained from the indicated yeast strains. A part of the Western blot membrane stained with Ponceau red is depicted as a loading control.