FIG 9.

The sphere of influence of Asc1p in a RACK wheel overview. The impacts of Asc1p in the adaptation of the cellular proteome and phosphoproteome are depicted schematically. Asc1p links cellular signal transduction to the ribosome (indicated in gray below the red Asc1p protein; the structure figure was generated with the PyMol Molecular Graphics System software on the basis of the PDB file 4V88), leading to adjustment of the cellular proteome as needed (indicated by red arrows). Simultaneously, Asc1p is important for the phosphorylation status of central components of various processes, named in the outermost ring of the scheme. Phosphorylations are illustrated with a “P” in a star. The inner ring around the central RACK wheel gives examples of proteins containing Asc1p-sensitive phosphorylation sites. The outer ring shows examples of proteins that are Asc1p-dependently regulated at the proteome level. Phosphorylation of Asc1p itself may modulate the protein's integrity as a link between translation and signal transduction. Additional references were used for the Flo11p (19), Kss1p (2), Scp160p (16), and Slt2p (29) data. HSP, heat shock protein; HXTs, hexose transporters.