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. 2017 Jan 19;37(3):e00535-16. doi: 10.1128/MCB.00535-16

FIG 1.

FIG 1

Ionizing radiation response in cells with and without MCL-1. (A) The left side shows Western blots representative of MCL-1 knockdown in three cell lines using MCL-1 siRNA, and the right side shows Western blot analysis of MCL-1 levels in control siRNA- and MCL-1 siRNA-treated cells transfected with plasmid pTOPO-MCL-1 to rescue the endogenous MCL-1 knockdown phenotype. (B) The Western blots represent the knockdown of the isoforms of MCL-1 (MCL-1 and MCL-1S) and expression of MCL-1S using pcDNA-MCL-1S. (C) Caspase 3/7 activity in control and MCL-1-depleted cells 48 h postirradiation. (D) Clonogenic survival after exposure of cells to graded IR doses. (a) DAOY cells; (b) H1299 cells; (c) U2OS cells; (d) clonogenic survival after irradiation of DAOY cells with 4 Gy of IR with and without knockdown of endogenous MCL-1 and ectopic expression of MCL-1; (e) clonogenic survival after irradiation of DAOY cells with 4 Gy of IR with and without knockdown of endogenous MCL-1 and MCL-1S and ectopic expression of MCL-1S.