FIG 7.
The WH motif and the HRDC domain of WRN contribute to cell proliferation and telomere length homeostasis. (A) Western blot analysis of WS-teli+dox parental cells and cells complemented with wild-type or mutant WRN proteins. +dox, cells grown in medium containing doxycycline; −dox, cells after doxycycline removal. (B) Analysis of proliferation of WS-teli+dox parental cells and cells complemented with wild-type or mutant WRN proteins after doxycycline removal. (C) TRF analysis of WS-teli+dox parental cells and cells complemented with wild-type or mutant WRN proteins before and after doxycycline removal. Average terminal restriction fragment lengths and standard deviations were calculated from the results of three independent experiments, as described in Materials and Methods.