Figure 3.

Electrophysiological recordings in brain slices demonstrating effects of GLP-1R signaling on ARC Kiss1 cells of OVX+E₂ and male mice. A, B, Current clamp recordings in ARC Kiss1 cells from brain slices treated with the long-acting GLP-1R agonist, liraglutide, showed membrane depolarization in both OVX+E₂ (A; 52% of cells responded) and males (B; 60% of cells responded). ***p < 0.001, ****p < 0.0001 vs. RMP, one-way RM-ANOVA with Bonferroni’s post hoc test; ^†††p < 0.001, 100 vs. 300 nm liraglutide, one-way RM-ANOVA with Bonferroni’s post hoc test. C, The magnitude of depolarization was greater in males compared with OVX+E₂ females at 100- and 300-nm concentrations. *p < 0.05, OVX+E₂ vs. males, one-way ANOVA with Bonferroni’s post hoc test. n = 13 male and 27 OVX +E₂ mice.