c. Viral RNA in spleens of infected mice (RT-PCR). | |||||||
---|---|---|---|---|---|---|---|
Strain | Dose in inoculum (PFU) | 5 h | 10 h | 28 h | |||
Detection*N = 3 | GMT** (log10 RNAs/ml) | Detection*N = 3 | GMT** (log10 RNAs/ml) | Detection*N = 3 | GMT** (log10 RNAs/ml) | ||
EK-328 | 103 | 0 | – | 0 | – | 0 | – |
106 | 3 | 4.0 ± 0.8 | 1 | 4.7 | 3 | 4.9 ± 0.2 | |
Absettarov | 103 | 0 | – | 0 | – | 0 | – |
106 | 3 | 5.4 ± 0.6 | 3 | 6.4 ± 0.2 | 3 | 4.2 ± 0.5 |
Mice were infected with cell culture grown virus stocks.
GMT–geometric mean titer.
N–total number of animals taken for experiment.
–the number of animals in which blood (brain, spleen) the virus was detected.
–in 10% tissue (brain, spleen) suspension.
“–” not detected.
Panel b–Real Time PCR sensitivity 1000 RNAs/sample.
Panel c–Real Time PCR sensitivity 3000 RNAs/sample.