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. 2017 Jan 20;7:2172. doi: 10.3389/fmicb.2016.02172
c. Viral RNA in spleens of infected mice (RT-PCR).
Strain Dose in inoculum (PFU) 5 h 10 h 28 h
Detection*N = 3 GMT** (log10 RNAs/ml) Detection*N = 3 GMT** (log10 RNAs/ml) Detection*N = 3 GMT** (log10 RNAs/ml)
EK-328 103 0 0 0
106 3 4.0 ± 0.8 1 4.7 3 4.9 ± 0.2
Absettarov 103 0 0 0
106 3 5.4 ± 0.6 3 6.4 ± 0.2 3 4.2 ± 0.5

Mice were infected with cell culture grown virus stocks.

GMT–geometric mean titer.

N–total number of animals taken for experiment.

*

–the number of animals in which blood (brain, spleen) the virus was detected.

**

–in 10% tissue (brain, spleen) suspension.

–” not detected.

Panel b–Real Time PCR sensitivity 1000 RNAs/sample.

Panel c–Real Time PCR sensitivity 3000 RNAs/sample.