FIGURE 1.

Expression levels of the YDR109C gene in the prototrophic yeast strains used in this study. Total RNA was extracted from exponentially growing cells of the WT and ydr109cΔ strains as well as the ydr109cΔ strain transformed with the p41Hyg 1-F::YDR109C plasmid (rescue) or the corresponding empty plasmid. The expression fold change of the YDR109C gene in the indicated strains relative to the WT strain was calculated using the 2^−ΔΔCt method. The expression level of the YDR109C gene in each sample was either normalized to reference gene ACT1 or ALG9. Means and standard deviations of three biological replicates are shown.