FIGURE 8.

Effect of PrPAP1 on melanization of P. rapae hemolymph. Ten microliters of diluted P. rapae hemolymph was mixed with M. luteus (0.5 μg), purified PpS1V, or 10 μl of culture medium containing activated PrPAP1 (cultured Sf-900 II medium harvested from eGFP-expressing recombinant baculoviruses was used as control). After incubation at room temperature for 5–15 min, PO activity was assayed as described under “Experimental Procedures.” All values are presented as the mean (n = 3). Error bars represent S.E. Asterisks indicate significant differences from the control. **, p < 0.01; ***, p < 0.001.