FIGURE 4.

Repeated intradermal administration of mMCP-8 induces leukocyte accumulation in the ear skin. C57BL/6 mice were challenged once or three times at 24 h-intervals with intradermal administration of 10 μg of mMCP-8, HI-mMCP-8, or control BSA in the ear skin. The ears of treated mice were excised 6 h after the last challenge. (A, B, and D) The number of CD45⁺ hematopoietic cells (A and D) and the indicated cell types (B) isolated from the ear skin are shown (mean ± S.E., n = 3–6 each). C, immunohistochemical analysis using anti-Ly-6B to detect neutrophils and a certain subset of monocytes/macrophages was performed. Scale bars = 200 μm. Data shown are representative of three independent experiments. Mo-Mac, monocytes/macrophages. *, p < 0.05; **, p < 0.01; ***, p < 0.001; n.s., not significant.