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. 2016 Dec 12;292(3):1081–1091. doi: 10.1074/jbc.M116.766741

FIGURE 5.

FIGURE 5.

The effect of TbNedd8 RNAi on FAZ filament assembly and flagellum attachment. A, percentage of cells with a detached flagellum and an snFAZ in control and TbNedd8 RNAi cells. Cells were immunostained with anti-FAZ1 antibody, and a total of 300 cells from each time point were counted. Error bars represent standard deviation from three independent experiments. ***, p < 0.001. d, day. B, assembly of the FAZ filament in control and TbNedd8 RNAi cells. Cells were immunostained with anti-FAZ1 antibody and counterstained with DAPI. The white arrows indicate the old FAZ filament, the solid white arrowheads indicate the new FAZ filament, and the open white arrowheads indicate the snFAZ. Black arrows show the detached flagellum. DIC, differential interference contrast. Scale bar = 5 μm.