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Perturbation of PDH bypass in crd1Δ cells. Wild-type and crd1Δ cells were grown in YNBA at 30 °C to the logarithmic phase. A, acetyl-CoA levels were determined as described under “Experimental Procedures.” Data shown are mean ± S.D. (n = 6) (**, p < 0.01). B, enzyme activity (units/mg) of acetyl-CoA synthetase was assayed as described under “Experimental Procedures.” Data shown are mean ± S.D. (n = 6) (***, p < 0.001).
