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. 2016 Dec 7;292(3):1103–1111. doi: 10.1074/jbc.M116.758367

FIGURE 2.

FIGURE 2.

Negative-ion MALDI-TOF-MS analysis of procyclins after removal of the GPI anchors. A and B, butan-1-ol extracts from WT (A) and Δrft1 (B) cells were subjected to 48% aqueous hydrofluoric acid dephosphorylation followed by mild trifluoroacetic acid hydrolysis to remove the GPI anchor and generate EP procyclin peptides. The resulting polypeptides, corresponding to the C-terminal portions of procyclins, were analyzed by negative-ion MALDI-TOF-MS. GP(−4) and GP(−13) refer to GPEET fragments lacking 4 and 13, respectively, amino acids at the N terminus. EP isoforms EP1-1 (I) (P(EP)nG-Etn) and EP1-1 (II) (PDP(EP)nG-Etn) represent C-terminal mild acid fragments (12). EP3-5 is an unusual form containing 21 EP repeats (49).