FIGURE 3.

Ectopic expression of TbRFT1 and TbRFT1-HA. EP and/or GPEET from WT cells, TbRFT1 null cells (Δrft1), and TbRFT1 null cells expressing a HA-tagged (+) or untagged (−) ectopic copy of TbRFT1 (Δrft1/RFT1) were analyzed by SDS-PAGE and immunoblotting. A and B, enhanced chemiluminescence was used to visualize HA (A) or EP (B) using corresponding first and secondary antibodies. C, EP and GPEET in WT, TbRFT1 null cells (Δrft1), and TbRFT1 null cells expressing a HA-tagged (+) or untagged (−) ectopic copy of TbRFT1 (Δrft1/RFT1) were labeled with [³H]ethanolamine, extracted, and analyzed as described in the legend for Fig. 1. The sizes of wild-type (solid lines) and mutant (dashed lines) proteins are marked (blue, EP; green, GPEET).