TABLE 3.
Comparison of thermodynamic parameters obtained from Trp fluorescence and SX
The parameters obtained by SX are extrapolated to 0 m for all FNIII domains studied. FNIII 2C*, FNIII 9C*, and FNIII 10C* were fit using a more complex mechanism (Equation 13). Trp(SVD) means the Trp fluorescence data were analyzed using singular value decomposition. S.E. values (see Table 1) are shown in parentheses.
| FNIII domain | Technique | ΔG | meq | C1/2 |
|---|---|---|---|---|
| kcal/mol | m | |||
| 1C | Trp | 6.1 (0.1) | −1.06 (0.17) | 5.7 (0.1) |
| 2C | SX | 5.9 (0.07) | −1.05 (0.02) | 5.6 (0.1) |
| 2C | Trp | 4.7 (0.4) | −0.81 (0.07) | 5.8 (0.04) |
| 3C | SX | 3.4 (0.03) | −1.39 (0.012) | 2.5 (0.03) |
| 3C | Trp | 3.3 (1.0) | −1.16 (0.34) | 2.8 (0.2) |
| 3C | Trp (SVD) | 3.8 (0.2) | −1.15 (0.07) | 3.4 (0.04) |
| 6C | SX | 5.6 (0.03) | −1.03 (0.0041) | 5.4 (0.05) |
| 6C | Trp | 3.4 (0.8) | −0.51 (0.16) | 6.7 (0.7) |
| 7 | SX | 5.9 (0.01) | −1.3 (0.02) | 4.6 (0.02) |
| 9C* | SX | 3.7 (0.01) | −1.0 (0.004) | 3.6 (0.02) |
| 9C | Trp | 3 (1.6) | −0.87 (0.44) | 3.4 (0.4) |
| 10C* | SX | 8.2 (0.009) | −0.93 | 8.8 |
| 10C | Trp | 10.6 (2.1) | −1.21 (0.24) | 8.8 (0.09) |
| 11C | SX | 0.78 (0.03) | −1.64 (0.002) | 0.48 (0.03) |
| 11C | Trp | 1.6 (0.7) | −1.79 (0.66) | 0.88 (0.1) |
| 12C | SX | 4.3 (0.02) | −1.45 (0.006) | 2.96 (0.02) |
| 12C | Trp | 5.4 (1.27) | −1.47 (0.34) | 3.7 (0.1) |
| Irisin | SX | 4.1 (0.01) | −1.42 (0.02) | 2.9 (0.01) |
| Irisin | Trp | 3.4 (0.8) | −1.41 (0.31) | 2.4 (0.1) |
| TNfn3 | SX | 2.9 (0.04) | −1.51 (0.03) | 1.9 (0.04) |
| TNfn3 | Trp | 2.5 (2.4) | −0.65 (0.6) | 3.9 (0.9) |