TABLE 5.

Amino acid sequences of the FNIII domains studied

The letters in lowercase type represent the His tag, the underlined letters represent the extra amino acids due to the cloning sites, and the native or introduced Cys is marked by bold and underline.

Domain	Amino acid sequence
FNIII 1C	(m)gsshhhhhhssglvprgshmHMTSSGPVEV…SYTCKGL…FDFTTTGT
FNIII 2C	(m)gsshhhhhhssglvprgshmHMTSSPLVAT…ATSCNID…STSQTTGT
FNIII 3C	(m)gsshhhhhhssglvprgshmHMTSAPDAPP…ANSCTLS…GTPRSDGT
FNIII 6C	(m)gsshhhhhhssglvprgshmHMTSLQPGSS…SGSCVVS…VNKVVTGT
FNIII 7	(m)gsshhhhhhssglvprgshmHMTSPLSPPT…QSSCTFD…SDTIIPGT
FNIII 9C	(m)gsshhhhhhssglvprgshmHMTSGLDSPT…RNSCTLT…IGQQSTGT
FNIII 10C	(m)gsshhhhhhssglvprgshmHMTSVSDVPR…KSTCTIS…SINYRTGT
FNIII 11C	(m)gsshhhhhhssglvprgshmHMTSEIDKPS…QTECTIE…VQTAVTGT
FNIII 12C	(m)gsshhhhhhssglvprgshmHMTSIPAPTD…SSSCVVS…VVTTLEGT
Irisin	(m)gsshhhhhhssglvprgshmSPSAPVN…TRSCALW…KDEVTMKE
TNfn3	(m)RLDAP…SLICRRG…ETFTT