Figure 4.

Depletion of the essential SSU processome proteins slows growth. Strains expressing Utp1-17 from a galactose-inducible/glucose-repressible promoter with a 3xHA tag were grown to early log phase in galactose/raffinose media (undepleted) and then shifted into glucose media (depleted) for 24 h. YPH499 (the parent untagged strain) was used as a control. After 0, 3, 6, 12, and 24 h of depletion, cells were analyzed for growth by OD₆₀₀ (A) and for protein expression by Western blotting with anti-HA antibodies (B).