Figure 6.

Immunofluorescence of depleted SSU processome proteins is consistent with G1 arrest. YPH499, GAL::3xHA-NET1, and GAL::3xHA-UTP18 yeast strains were grown to early log phase in galactose/raffinose media (undepleted) and then shifted into glucose (depleted) for 23 h. YPH499 (the parent untagged strain) and GAL::3xHA-NET1 (a protein required for mitotic exit) were used as controls. Immunofluorescence was carried out using antibodies to tubulin and detected with TRITC (green), and Mpp10 was detected with FITC (red). DAPI (blue) was used to stain the cellular DNA.