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. 2017 Jan 20;7:41058. doi: 10.1038/srep41058

Figure 4. miR-27a was transferred from VSMCs to ECs via VSMC-MPs.

Figure 4

(a,b) VSMCs were exposed to static condition, 5% and 15% cyclic stretch for 24 h, respectively, and the VSMC-MPs obtained from each CM were used to stimulate ECs for 24 h. The expression of miR-27a (a) and the expression of pre-miR-27a (b) in ECs were detected by qPCR. ‘----’ indicates the static values standardized to 1. The values are shown as the mean ± SD for each condition from at least four independent experiments. *P < 0.05 vs. 5%. (c) VSMCs were transfected with B-miR-27a at a concentration of 30 nmol/L for 24 h, and the negative control was transfected with miR-27a without biotin labeling. VSMC-MPs from these VSMCs were isolated to treat ECs. B-miR-27a in the VSMCs and ECs was tracked using rhodamine-labeled streptavidin and detected by immunofluorescence. The six top images show the successful transfection of B-miR-27a in the VSMCs, and the six bottom images show the transfer of miR-27a from VSMCs to ECs. Scale bar = 20 μm.