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. 2017 Jan 20;7:41058. doi: 10.1038/srep41058

Figure 5. The effect of miR-27a and GRK6 on the proliferation of ECs.

Figure 5

(a,b) ECs were transfected with miR-27a mimics and inhibitor at a concentration of 100 nmol/L for 48 h. (a) PCNA expression of ECs was detected by western blotting. (b) Proliferation of ECs was detected by BrdU ELISA. (c–e) ECs were transfected with GRK6-targeting siRNA at a concentration of 100 nmol/L or Lv-GRK6 for 48 h. (c) GRK6 expression and (d) PCNA expression of ECs were detected by western blotting. (e) Proliferation of ECs was detected by BrdU ELISA. The values of control groups were standardized to 1 and the values are shown as the mean ± SD for each condition from at least four independent experiments. *P < 0.05 vs. control. Full-length blots are presented in Supplementary Fig. S7.