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. 2017 Jan 20;7:41090. doi: 10.1038/srep41090

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Cells were treated with 100 μg/mL MHG-FA or DOX-loaded MHG-FA for 30 min and further incubated for 48 h. Apoptosis was analyzed by Annexin V/7-AAD double staining and flow cytometry. (a) Representative results from three independent experiments. The lower right quadrant represents early apoptotic cells stained mainly by Annexin V and the upper right quadrant stands for late apoptotic cells stained by both Annexin V and 7-AAD. (b) The percentages of both apoptotic (late and early) and necrotic cells induced by MHG-FA and DOX-loaded MHG FA.