Figure 3.

Specific interactions between tobacco LTP1 and high-affinity sites located on tobacco plasma membranes. Plasma membrane preparations were incubated with various concentrations of ¹²⁵I-labelled LTP1. Specific binding was determined by subtracting nonspecific binding from total binding. Experiments were repeated at least five times, and results are expressed as the mean values ± SD (fmol bound LTP1/mg plasmalemma proteins). •, LTP1; ▴, LTP1-LA; and ▵, LTP1-JA. Inset: displacement of ¹²⁵I-labelled LTP1 specifically bound to plasma membrane by lysozyme (A), unlabeled LTP1 (B), or unlabeled cryptogein (C), 30 min after the addition of the unlabeled protein. The experiments were repeated three times, and results are the mean values ± SD.