Figure 4.

Level of tobacco resistance to P. parasitica induced by cryptogein and tobacco LTP1. Ethanolic elicitor solutions were applied on the stem section of freshly decapitated plants (cryptogein 0.1 nmol/plant, LA or JA 1 nmol/plant, LTP1 or LTP1-LA or LTP1-JA 0.5 nmol/plant). Ethanol (1%) was added on control plants. Twenty-four hours later, a mycelial plug was dropped on the cut petiole of the third fully expanded leaf from the apex. Seven days after inoculation, stems were split in two, and the internal necroses, corresponding to the brown or desiccated zones, measured. Results are expressed as the means of percentage of protection ± SD from three replicate experiments and five plants for each treatment. Small letters represent the result of a Student-Newman-Keuls test at 0.05.