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. 2017 Jan 20;7:40968. doi: 10.1038/srep40968

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Copyright © 2017, The Author(s)

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Effects of subcutaneously injected BIIE0246 (0.03 mmol/kg) and intraperitoneally injected PYY(3–36) (0.2 mg/kg) on pain evoked by intrarectally administered PO or AITC (2%, 0.05 ml) in C57BL/6 N mice. Shown are pain-related behaviors (A), time spent freezing (B), latency to first pain-related behavior (C), and time spent grooming (D). The behavioral parameters were evaluated blindly from 15-min videos recorded immediately after intrarectal treatment. One-way ANOVA with Welch’s correction revealed significant differences among the groups. The data shown are means + SEM, n = 6 for PO groups and 9–12 for AITC groups; *p < 0.05, **p < 0.01, ***p < 0.001 compared to vehicle + PO, ^#p < 0.05 compared to vehicle + AITC.