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. 2017 Jan 20;7:41129. doi: 10.1038/srep41129

Figure 4. Expression of apoptosis-related genes in the cerebral cortex of mice following chronic RF-EMF exposure.

Figure 4

(A) The cerebral cortical RNA extracted sham-exposed and RF-exposed mice were analysed for the expression level of autophagy genes by quantitative real-time PCR. (a,b) Quantification of Bcl2 and Bax mRNA transcripts by qRT-PCR. (c) 1.5% Agarose gel electrophoresis showing differential expression of Bcl2 and Bax by sqRT-PCR. The expression values of the cerebral cortex of RF-exposed mice were normalized to those of the sham-exposed mice. The relative mRNA levels of each gene were calculated by normalizing to expression of GAPDH by the 2−ΔΔCt method (n = 6). (B) (a) Total protein was subjected to 15% SDS–PAGE and immunoblotted with antibodies against Bcl2 and Bax. α-tubulin was used as loading control. (b) The intensity of bands of western blot was quantified by densitometry. The protein level was normalized relative to α-tubulin. Each bar represents the mean ± SEM of three independent experiments. Statistical significance was evaluated using a t-test: *P < 0.05, **P < 0.01.