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. 2017 Jan 20;7:40984. doi: 10.1038/srep40984

Figure 1. ESAT-6 stimulates IL-6 production by macrophages.

Figure 1

Mouse BMDMs (A) were treated with or without ESAT-6 as indicated and IL-6 levels in the culture supernatants were determined after 24 h incubation. (B) BMDMs were incubated with or without ESAT-6 at 1 μg/ml for various times and IL-6 levels were determined. BMDMs (C), mouse alveolar macrophages (D) and RAW 264.7 cells (E) were incubated with indicated concentrations of proteins and IL-6 levels after 24 h incubation were determined. (F) BMDMs treated with or without 10 μg/ml polymyxin B (Poly. B) for 1 h prior to incubation with medium alone, ESAT-6 (1 μg/ml), LPS (0.1 μg/ml) or Pam3CSK4 (0.1 μg/ml) for 24 h and IL-6 levels were determined. For all panels, data are expressed as means ± SEM, *P < 0.05, **P < 0.01 or ***P < 0.001. n.s., not significant.