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. 2017 Jan 20;7:41043. doi: 10.1038/srep41043

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This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a–c) Confocal images of wild-type sibling and Tg(myl7:EGFP-HsHRAS)^s883 isl1−/− embryos stained with anti-GFP and anti-Isl1/2 antibodies at 24 somites (a), 26 hpf (b) and 48 hpf (c). Arrows point to Isl1⁺ cells at the periphery of the cone (a) or Isl1⁺ cardiomyocytes at the venous pole of the atrium (b,c), arrowheads point to residual Isl1/2⁺ cardiomyocytes in the future ventricle (a) or the inner curvature of the ventricle and the outflow pole (b,c). Scale bars, 50 μm.