Table 1.
No. of metaphasesa
|
No. of anaphases
|
|||||||
---|---|---|---|---|---|---|---|---|
Genotype | No. of cells scored | Diploid | Polyploid | Diploid | Polyploid | Polyploid figures (%) | Anaphases (%) | Mitotic indexb |
dck1/dck1 | 254 | 81 | 127 | 19 | 27 | 60.6 | 18.1 | 0.68 |
dck2/dck2 | 273 | 152 | 77 | 38 | 6 | 30.4 | 16.1 | 0.55 |
dck2/Df(3L)E44 | 373 | 131 | 169 | 42 | 31 | 53.6 | 19.6 | 0.94 |
dck3/Df(3L)E44 | 385 | 272 | 48 | 55 | 10 | 15.1 | 16.9 | 0.76 |
Oregon R (control) | 282 | 227 | 0 | 55 | 0 | 0 | 19.5 | 0.70 |
This class includes both prometaphases and metaphases
The mitotic index corresponds to the average number of divisions per optic field. The optic field used in this analysis is the circular area seen under a phase contrast Neofluar 100× oil-immersion Zeiss objective, by using 10× oculars and the Optovar set at 1.25