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. 2017 Jan 20;7:41224. doi: 10.1038/srep41224

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Corneal Epithelial Cells Cultures Isolated from (A) NWT and (B) NKO mice were Positively Stained with Corneal Epithelial Specific Marker, Cytokeratin K3 on Immunofluorescence Staining. (A) Staining for NWT culture. (i) cytokeratin K3 (K3), (ii) DAPI, (iii) combination of K3 with DAPI. (iv) cytokeratin K19 (K19), (v) DAPI, (vi) combination of K19 with DAPI. NWT cell cultures were positively stained with K3 (i, ii, iii), and negatively stained with K19 (iv, v, vi) (NWT, N = 3; NKO, N = 3. (B) Staining for NKO culture. (i) K3, (ii) DAPI, (iii) combined K3 and DAPI. (iv) K19, (v) DAPI, (vi) combined K19 with DAPI. NKO mice cell cultures were positively stained with K3 (i, ii, iii) and negatively stained with K19 (iv, v, vi) (NWT, N = 3; NKO, N = 3). Our data confirmed that the isolated cultures from NWT and NKO mice were (~100% K3-positive) pure corneal epithelial cells.