Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 20;7:41224. doi: 10.1038/srep41224

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Cells were transfected with luciferase reporter and the corresponding luciferase activity represented intracellular NFκB/ISRE activity. Next, the NUCKS expressing construct (NUCKS) or empty vector (Control) was transfected to the cells, followed by (A) TNFα treatment or no treatment (UT); NFκB activation was measured (B) lipopolysaccharide (LPS) or no treatment (UT); (i) NFκB activation was measured and (ii) interferon stimulated response element (ISRE) activation was measured. (C) Poly-inosinic-cytidylic (polyIC) treatment or no treatment (UT); (i) NFκB activation was measured and (ii) interferon stimulated response element (ISRE) activation was measured. (D) On NUCKS silencing and LPS-induced-NFκB activation, the level of expression of (i) TNFα, (ii) IL8 and (iii) MCP1 was tested. (E) On NUCKS silencing and LPS-induced-NFκB activation, protein expression level of the phosphorylated form of IKK, P65 and IκBα was analyzed.