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. 2017 Jan 20;7:41040. doi: 10.1038/srep41040

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(a) A graphical representation showing gene deletion of oah in A. niger and primers used for validation of recombinated genome. (b) PCR products of the sequences down-stream of oah using primers P3 and P4 (upper) and oah gene using primers P1 and P2 (below). Lane 1–20, A. niger transformants; Lane CK, wild type of H915-1; Lane M, DNA ladder. (c) Citrate and oxalate production of H915-1 and H915 (Δoah::hph).