Figure 1. Frequency and amplitude of mEPSCs in the lateral habenula (LHb) are higher in the afternoon than in the morning.
(A) mEPSCs frequencies obtained during ZT3–12 showed a significant variations depending on the time of recording (F(8, 219) = 3.46, p < 0.001 by one-way ANOVA test). The dominance of high frequency (>7 Hz) cell was increased in ZT8–12 than ZT3–7 (p < 0.05, χ2 test). (B) The mean mEPSCs amplitude per each hour during ZT3–12 remained comparable across the time point of recordings (F(8, 219) = 1.05, p > 0.3 by one-way ANOVA test). The dominance of large amplitude (>30 pA) cell was increased in ZT8–12 than ZT3–7 (p < 0.05, χ2 test). (C) mEPSC frequency was 1.87 ± 0.34 Hz (63 cells from 11 animals) during ZT2–6 (sacrificed at ZT1; ZT2(ZT1) group) and 2.88 ± 0.34 Hz (52 cells from 8 animals) during ZT8–12 (sacrificed at ZT1; ZT8(ZT1) group) (p < 0.05 by t-test, p < 0.05 by bootstrap). The box-plot shows the range of mEPSC frequencies obtained in each group. (D) The average mEPSC amplitude of the ZT2(ZT1) group was 20.14 ± 0.97 pA and the average of the ZT8(ZT1) group was 21.45 ± 1.63 pA (p > 0.47 by t-test, p > 0.4 by bootstrap). The box-plot shows the range of mEPSC amplitudes obtained. Representative traces are shown at the top of each panel (scale bars: 1 s and 40 pA).