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. 2017 Jan 20;7:40778. doi: 10.1038/srep40778

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(A) HT29 or U2OS cells were treated with indicated combinations of metabolism modulator with either 0 or 0.4 μM Chk1i for 24 hours. The fraction of nuclei scored positive for γH2AX or pChk1 (S317) along with the mean nuclear intensity of the positive cells was determined using single cell immunofluorescent imaging (n = 4, mean ± SD). Dotted lines indicate Chk1i single-agent activity. (B) Cell lysates prepared from HT29 (upper) or U2OS (lower) cells treated with the indicated metabolism modulators for 24 hours were immunoblotted using the indicated antibodies (n = 1).