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. 2017 Jan 20;7:40778. doi: 10.1038/srep40778

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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HT29 cells were treated with the indicated metabolism modulators for 24 hours then EdU for a further 15 minutes. DNA content, EdU incorporation and pHH3 (S10) expression was determined using single cell immunofluorescence analysis. (A) Single cell plots of relative DNA content versus total nuclear EdU demonstrating the different cell cycle populations. (B) Quantification of cell populations positive for EdU incorporation or pHH3 (S10) expression (n = 3, mean ± SD). (C) Determination of cell cycle distribution based on total nuclear DNA content (n = 3, mean). (D) Cell lysates prepared from HT29 cells treated with the indicated metabolism modulators were immunoblotted using the indicated antibodies (n = 1).