Figure 4. NSP family sequence conservation mapping to the insect allergen repeat structure.

Residue conservation is colored from red (invariant) to blue (variable). The NSP N-terminal ( A), middle ( B), and C-terminal ( C) domain repeats are represented in ribbon (upper panels) and surface (lower panels). Lipids from the insect allergen structure (4jrb) are in magenta sticks. ( D) Zoom into the phospholipid binding site (N-terminal domain), with the head group colored by atom: P (orange), O (red), and C (black). The larger side group of the phospholipid ligand (white) is not compatable with the NSP YxxxW ₁₈₇ motif (shown in stick). ( E) Comparison of phospholipid ligand (stick representation) with aglycone, with similar atom backbone orientations boxed. Sequence conservations were calculated using Al2CO ⁷² from an alignment of the following: Pieridae NSP1 and NSP2, together with AAR84202.1, ABY88944.1, ABX39547.1, ABX39554.1, ABY88945.1, ABX39555.1, ABX39546.1, ABX39549.1, ABX39537.1, ABX39552.1, ABX39553.1 from the NCBI Non-redundant protein database.