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. Author manuscript; available in PMC: 2017 Jan 20.
Published in final edited form as: Cell Rep. 2016 Dec 20;17(12):3178–3192. doi: 10.1016/j.celrep.2016.11.077

Figure 4. Notch-activated ESC-HSCs Reconstitute Definitive Erythroid Lineages.

Figure 4

Derivation of ESC-RBCs and BM erythroid progenitors from iICN ESC-HSCs. (B) Erythroid reconstitution of donor CD45.2+ BMs from NSGs transplanted with ESC-HSC-OP9/DL1 at 23 wks post-transplant. (C) Sorting scheme of ESC-HSC-DL1-derived erythroid BM progenitors (red) at 37 wks post-secondary transplant. (D–E) Transcript levels of β-Major, β-H1, ΕY, Bcl11a, as well as globin composition of each biological replicate of B6 BM and E14.5 FL (Ter119+), E10.5 blood (CD71+ Ter119+), ESC-RBC-OP9/DL1 (GFP+ Ter119+) and engrafted ESC-HSC-DL1-derived erythroid progenitors. See also Fig.S4.