Table 1.
16S rRNA gene sequencing analysis and culture method results for the environmental survey of the northern ward
| The northern ward | Samples | 16S rRNA analysis | Sequence length1) | %, Identity2) | Culture method |
|---|---|---|---|---|---|
| Nurses’ station | System of urinal collection |
Corynebacterium sp. Propionibacterium sp. Neisseria sp. Staphylococcus sp. Actinomyces sp. |
880 810 786 818 801 |
100 99 99 100 99 |
(−) |
| Personal computer |
Acinetobacter
sp.
Staphylococcus sp. Corynebacterium sp. Propionibacterium sp. |
810 797 801 794 |
99 99 99 100 |
(−) | |
| Faucet |
Acinetobacter
sp.
Serratia sp. Staphylococcus sp. |
884 790 806 |
99 99 99 |
(−) | |
| Outlet |
Acinetobacter
sp.
Stenotrophomonas sp. |
816 791 |
99 99 |
Enterobacter cloacae
Stenotrophomonas maltophilia |
|
| Patient’s room 1 | Fence of the bed |
Staphylococcus sp. Propionibacterium sp. Corynebacterium sp. |
884 802 817 |
99 99 99 |
CNS |
| Aspirator |
Acinetobacter
sp.
Corynebacterium sp. Staphylococcus sp. Neisseria sp. Bacteroides sp. Prevotella sp. Actinomyces sp. |
802 794 793 798 819 800 786 |
99 99 99 100 99 99 99 |
(−) | |
| Trash box |
Staphylococcus sp. Stenotrophomonas sp. Corynebacterium sp. |
805 789 814 |
99 99 99 |
CNS Klebsiella pneumoniae Asperugirus sp. |
|
| Patient’s room 2 | Fence of the bed |
Acinetobacter
sp.
Corynebacterium sp. Klebsiella sp. Enterocococcus sp. Fusobacterium sp. Lactobacillus sp. Prevotella sp. |
823 820 746 738 805 812 794 |
99 99 99 99 99 99 99 |
(−) |
| Intubation tube |
Acinetobacter
sp.
Corynebacterium sp. Staphylococcus sp. Stenotrophomonas sp. Fusobacterium sp. Lactobacillus sp. |
805 882 797 806 802 802 |
99 99 99 99 99 99 |
Acinetobacter baumannii | |
| Patient’s room 3 | Fence of the bed |
Staphylococcus sp. Corynebacterium sp. Actinomyces sp. |
844 889 820 |
99 99 99 |
CNS |
| Intubation tube |
Acinetobacter
sp.
Staphylococcus sp. Stenotrophomonas sp. |
804 836 801 |
99 99 99 |
Acinetobacter lwoffii
CNS |
|
| Patient’s room 4 | Outlet | Stenotrophomonas sp. | 803 | 99 |
Aeromonas hydrophia
Stenotrophomonas maltophilia |
| Curtain |
Staphylococcus sp. Streptococcus sp. Neisseria sp. Corynebacterium sp. Escherichia sp. Fusobacterium sp. |
773 771 801 798 803 800 |
99 99 99 99 99 99 |
(−) | |
| Dirty utility room | Urinal surface | Escherichia sp. | 823 | 99 | (−) |
CNS coagulase-negative staphylococci
1)Length of sequence used in the Basic Local Alignment Search Tool (BLAST) search
2)Based on BLAST alignment