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. 2017 Jan 19;17:24. doi: 10.1186/s12862-016-0863-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Quantitative changes of amphioxus CYP26 expression in response to retinoic acid (RA) signaling alterations. Expression of CYP26-1, CYP26-2, and CYP26-3 in amphioxus (Branchiostoma lanceolatum) was assessed by quantitative real time PCR (qPCR) at two developmental stages (mid neurula at 20 h of development and early larva at 48 h of development). RAR expression was also established and used as a normalized positive control. (a) Relative normalized expression (left panel) and fold change of expression levels (right panel) at the mid neurula stage, using a Log₂ scale. (b) Relative normalized expression (left panel) and fold change of expression levels (right panel) at the early larval stage, using a Log₂ scale