FIG. 6.
Evaluation of HS2G NN-ELISA to identify serum samples from uninfected, infected, and vaccinated horses. The panel of reference serum samples included uninfected horses (samples 1 to 7), horses persistently infected with EIAV (samples 8 to 25), and horses vaccinated with attenuated S2 vaccine strains (samples 26 to 46). All horse sera were used at a dilution of 1:25 in HS2G NN-ELISA. The affinity ELISA procedures for detecting serum antibodies to the HS2G antigen or the EIAV p26 protein were as described in Materials and Methods.