Figure 3.

The pen1-1 syp122-1 double mutant is severely dwarfed and necrotic. (A, C, D, E [left], and G) double mutant; (B, E [right], and F) wild-type. (A) Necrotic lesions in the double mutant grown under sterile conditions on plates. (B-D) Roughly 3-wk-old soil-grown seedlings. (B and D) High light (c. 400 μE m^-2 s^-1); (C) low light (c. 100 μE m^-2 s^-1). The seedling grown under low light in C has fewer lesions and is larger than the one in D, grown under high light. (E) Note the dwarfed phenotype of the double mutant (left) compared with the wild-type (right). (F and G) Electron micrographs of wild-type (F) and double mutant (G) seedlings show the presence of two cuticular layers, a dark layer of cutin interwoven with cell wall polymers (star) and a white layer of cutin. Above the cuticle at the leaf surface is an omiophillic layer that may correspond to epicuticular waxes, partially removed (see especially F) by solvent extraction during sample preparation.