Table 2.
Plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| pRS304 | TRP1 (integrative) | Sikorski and Hieter (1989) |
| pRS305 | LEU2 (integrative) | Sikorski and Hieter (1989) |
| pRS424 | TRP1 (high copy) | Christianson et al. (1992) |
| pRS426 | URA3 (high copy) | Christianson et al. (1992) |
| YEplac181 | LEU2 (high copy) | Gietz and Sugino (1988) |
| YEplac195 | URA3 (high copy) | Gietz and Sugino (1988) |
| p98 | axl1::URA3 (insertional knock-out construct) | Adames et al. (1995) |
| YEpHA-BUD8F | HA3-BUD8 in YEplac181 | Schenkman et al. (2002) |
| YEpGFP-BUD8F | GFP-BUD8 in YEplac181 | Schenkman et al. (2002) |
| pCA5015 | 1.7-kb BamHI fragment containing the HIS3 gene in pBluescript-KS(+) BamHI site | T.-H. Chang |
| pHP835 | BUD5-HA6 in YCp50 | Kang et al. (2001) |
| pHP896 | 1.9-kb RAX1 in pBluescript-SK(+) | See text |
| pHP898 | rax1Δ::HIS3 in pBluescript-SK(+) | See text |
| pHP899 | 2.6-kb RAX1 in pBluescript-SK(+) | See text |
| pHP924 | rax1Δ::URA3 in pBluescript-SK(+) | See text |
| pHP926 | pBluescript-SK(+) with NotI site destroyed | This study |
| pHP933 | RAX1 with N-terminal NotI site in pHP926 | See text |
| pHP995 | rax2Δ::HIS3 in pBluescript-SK(+) | See text |
| pHP996 | 4.2-kb RAX2 in pBluescript-SK(+) | See text |
| pHP1012 | RAX2 with C-terminal NotI site in pBluescript-SK(+) | See text |
| pHP1013 | RAX2 with C-terminal NotI site in pRS304 | See text |
| pHP1016 | RAX2-HA3 in pRS304 | See text |
| pHP1017 | RAX2-GFP in pRS304 | See text |
| pHP1061 | GFP-RAX1 in pRS304 | See text |
| pHP1090 | RAX2-HA3 in pRS305 | See text |
| pHP1091 | RAX2-GFP in pRS305 | See text |
| pHP1102 | RAX2-HA3 with additional 5′ sequences in pRS305 | This studya |
| pHP1109 | GFP-RAX1 in pRS305 | See text |
| pHP1110 | rax2Δ::URA3 in pBluescript-SK(+) | See text |
| pHP1156 | pGAL1p-GST-HIS6-RAX1 | Zhu et al. (2001) |
| pHP1158 | pGAL1p-GST-HIS6-RHO5 | Zhu et al. (2001) |
| pHP1159 | pEGFP-C3 with NotI sites | See text |
| pHP1169 | AXL2-Protein C (C-terminus) in pRS424 | This studyb |
| pHP1183 | RAX2-HA3 in pRS426 | This studyc |
| pHP1202 | GFP3-BUD9 in YEplac195 | See text |
| pHP1319 | HA3-BUD9 in YEplac181 | This studyd |
a
Contains 1350 bp of RAX2 upstream sequences.
b
The 4-kb NsiI fragment from YEp24 AXL2-Protein C (Kang et al., 2001) was ligated into the PstI site of pRS424.
c
The 4.8-kb HindIII-XhoI fragment from pHP1102 was ligated into the HindIII and XhoI sites of pRS426.
d
The 2.7-kb SphI fragment from YEpHA-BUD9 (Harkins et al., 2001) was ligated into the SphI site of YEplac181.