Figure 3.

HCP-1/CeCENP-F dissociates from the centromere during prometaphase. A prometaphase nucleus from a four-cell embryo was stained with DAPI (blue) and α-HCP-3 (green) and α-HCP-1 (white [b] or red [c]) antibodies. At this stage in mitosis, fully resolved centromeres can be observed on all the chromosomes (a). HCP-1 has maintained associations with some of the centromeres (arrows in c), but it is also present in a diffuse sphere around the chromosomes (b and c), indicative of an intermediate stage in its reorganization away from the centromere. Bar, 1 μm.