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. 2004 Oct 25;101(44):15650–15655. doi: 10.1073/pnas.0308088101

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Copyright © 2004, The National Academy of Sciences

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Fig. 6. — Interaction of torsinA and ΔE-torsinA. (A). Cross-linking of torsinA (Upper) and ΔE-torsinA (Lower). HEK293 cells expressing either WT-torsinA or ΔE-torsinA were incubated with increasing concentrations of DSP as described in Materials and Methods. Immunoblotting was performed with a polyclonal anti-torsinA antibody. (B) Coimmunoprecipitation between Flag-tagged torsinA and GFP-tagged torsinA, Flag-tagged torsinA and GFP-tagged ΔE-torsinA, and Flag-tagged ΔE-torsinA and GFP-tagged ΔE-torsinA. Immunoprecipitation was performed with the Flag antibody and immunoblotting with the torsinA antibody. TA, torsinA; ΔETA, ΔE-torsinA. (C) Dominant-negative effect of ΔE-torsinA on wild-type torsinA distribution. TorsinA was tagged with the Flag epitope and stained with the Flag antibody, followed by a Texas red secondary antibody (red). ΔE-torsinA was tagged with GFP (green).