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. 2004 Oct 20;101(44):15748–15753. doi: 10.1073/pnas.0403812101

Fig. 2.

Fig. 2.

Expression of CD209L protein by CHO cells transduced with a human lung cDNA library. (A) Flow cytometric analysis of clone 2.27 cells with antibodies specific for CD209L and DC-SIGN. Antibodies specific for DC-SIGN, CD209L, and an antibody that recognizes both DC-SIGN and CD209L were used to determine which glycoprotein was expressed on clone 2.27 cells. Only the antibodies that recognized CD209L bound to the clone 2.27 cells. (B) Immunoblots for ACE2 or CD209L of cell lines used in this study. Cell lysates from CHO cells, clone 2.27 cells, CHO cells stably expressing CD209L, CHO cells transiently transfected (Tf) with CD209L, and Vero E6 cells were run on a 10% SDS/PAGE gel and blotted onto poly(vinylidene difluoride). The blots were probed with polyclonal antibodies specific for the N or C termini of CD209L or for ACE2. The antibodies were detected by horseradish peroxidase-conjugated donkey anti-goat IgG antibodies and chemiluminescence.