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Macrophage activity in naïve, Tm-infected, and HH-Tm TLR2+/+ and TLR2−/− mice. Peritoneal adherent cells were cultured in the presence or absence of TmExo and, after 24 h, the levels of hydrogen peroxide (H2O2), TNF-α, and IL-1β were determined in cell-free supernatants. (A) TLR2+/+ and TLR2−/− naïve mice, (B) Tm-infected TLR2+/+ and TLR2−/− mice, (C) HH-Tm TLR2+/+ and TLR2−/− mice. Results are expressed as means ± SEM. (Unpaired t-test; *P < 0.05, **P < 0.01, ***P < 0.001; n = 5–6).
