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. 2004 Oct 20;101(44):15597–15602. doi: 10.1073/pnas.0405238101

Fig. 5.

Fig. 5.

ChIP assay of rat and mouse liver nuclear extract. Intact nuclei isolated from liver of rat and mouse fed a high-starch diet were treated with formaldehyde to crosslink DNA with associated DNA-binding proteins. Chromatin fragments immunoprecipitated with ChREBP antibodies (Ch-X) and purified input DNA were amplified by PCR with primers for rat LPK, rat (r) FAS, rat albumin, mouse (m) ACC, and mouse albumin gene promoters, and the reaction products were separated on a 10% polyacrylamide gel. Immunoprecipitation of noncrosslinked DNA with anti-ChREBP (Ch-NX) and immunoprecipitation with normal serum (NS-X) were performed as controls.