Fig. 7.

BET2 knockdown in N9 microglial cells inhibits inflammatory cytokine expression. N9 cells were infected with lentivirus expressing BET2 or BET4 siRNAs for 3 days and then GFP-positive (infected) cells were purified by flow sorting and cultured for 2–3 days. The cells were either used for Western blotting or stimulated with LPS (1 μg/ml) for 2 h and then subjected to qRT-PCR. a, b Western blots showing BET protein knockdown. Quantification: mean ± SEM; n = 3 experiments; *P < 0.05, **P < 0.01 compared to scrambled siRNA. c qRT-PCR determination of inflammatory cytokine mRNAs. Quantification: mean ± SEM; n = 3 experiments; *P < 0.05, **P < 0.01 compared to scrambled siRNA + LPS