Table 1. Regulation of gene expression in epiblast explants.
| Gene | Staining | None | Mock | Ndl | pre-Ndl |
|---|---|---|---|---|---|
| NodallacZ | + | 12 | 1 | 6 | |
| ++ | 2 | 6 | 0 | ||
| +++ | 2 | 6 | 0 | ||
| Fgf4 | - | 6 | 6 | 11 | 3 |
| + | 0 | 0 | 4 | 4 | |
| ++ | 0 | 0 | 8 | 7 |
Numbers correspond to the total of explants from two to four independent whole-mount in situ stainings that were recovered after treatment with the factors indicated (top row). Processed Nodal (Ndl), uncleaved precursor (pre-Ndl), or conditioned medium of untransfected HEK293Tcells (mock) were applied as described in Materials and Methods. The relative intensities of staining (-, +) were scored visually.