Table 1.

Bacteriological results of all samples collected in both the herds of primiparous Mediterranean buffalo during the first 90 days in milk of investigation. Percentages represent the proportion calculate on the total number bacteriological results

	Herd A				Herd B
	VG1		N-VG1		VG2		N-VG2
Pathogens	No.	Percent	No.	Percent	No.	Percent	No.	Percent
Staphylococcus aureus	49	30.2	52	32.5	55	41.9	61	50.4
Coagulase-negative staphylococci	32	19.9	32	19.3	41	31.3	16	13.1
Enterococcus faecalis	2	1.2	2	1.3	1	0.8	1	0.8
Aerococcus viridans	4	2.5	4	2.5	2	1.5	4	3.3
Streptococcus dysgalactiae	2	1.2	1	0.6	0	0	2	1.7
Streptococcus thoraltensis	2	1.2	2	1.3	0	0	1	0.8
Other gram-positive pathogens (not S. aureus)	2	1.2	5	3.1	3	2.3	2	1.7
Escherichia coli	0	0	0	0	1	0.8	2	1.7
Acinetobacter lwoffii	4	2.5	3	1.9	0	0	2	1.7
Bacillus spp.	0	0	0	0	0	0	1	0.8
Culture negative	65	40.1	59	36.9	28	21.4	29	24.0
Total	162	100	160	100	131	100	121	100

No = number; VG1 and N-VG1 = Vaccinate Group1 and Not-Vaccinated Group1, respectively; VG2 and N-VG2 = Vaccinate Group1 and Not-Vaccinated Group2, respectively. Herd A: received two doses of vaccine pre-calving; Herd B: received two doses of vaccine pre-calving and one post-calving