Fig. 6.

ChACE2 and ChSSD1 are required for full virulence of C. higginsianum. a Schematic representation of the genomic loci of ChACE2 and ChSSD1 (top) and the knockout cassettes of the respective deletion plasmids (bottom). The position of 5’ and 3’ flanking regions are given relative to the respective start codon. b Spray infection of A. thaliana at 3 dpi. Primary hyphae (relative to appressoria) and secondary hyphae (relative to primary hyphae) produced by the indicated strains. c Vegetative growth of the indicated strains on potato dextrose agar (PDA) and Czapek-Dox agar minimal medium (MM). d Average number of conidia (left) and cell volume (right) for the indicated strains recovered from oatmeal agar after 7 days. The data from 3 separate plates is shown. The strains used in b-d were wild-type (CY5535), vir-88, ΔChace2 (CY6353), ΔChssd1 (CY6649) and the ΔChku80 parental strain of the last two (CY6021). Error bars are represented as the standard error from three replicates. Significant differences based on t-tests (p < 0.05) are marked with asterisks