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. 2004 Oct 26;101(44):15567–15572. doi: 10.1073/pnas.0406901101

Fig. 6.

Fig. 6.

Preliminary fit of TE (green backbone trace) and KSI (maroon trace) in the 20-Å resolution cryo-EM mass density. The substructures seen from the density are designated Head, Torso, and Foot. The TE trace fits best in the substructure designated Foot, whereas the KSI backbone trace fit is best in substructure designated Head. The best fit for both KSI and TE is circled in red. The backbone traces of TE and KSI at the bottom of the mass density show less satisfactory fits in the head and foot, respectively. Based on prior proteolytic digestion experiments of intact FAS, three domains were identified: domain I, which comprises the KS-AT/MT-DH enzyme centers and the linker region; domain II, which comprises the ER-KR-ACP region; and domain III, which is assigned to the TE (1, 2). The Head and Torso substructures, which are essentially coalesced, could correspond to domain I and parts of domain II, and the Foot could represent the rest of domain II and domain III.